[Antifungal activity of mouthwashes against Candida albicans and Rhodotorula mucilaginosa: An in vitro study]. / Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: un estudio in vitro.

BACKGROUND: Candida albicans and Rhodotorula mucilaginosa are yeasts of clinical importance in the oral cavity. In immunocompromised patients they can cause some pathologies that must be controlled with antimicrobials. AIMS: To evaluate and compare the antimicrobial efficacy of commercially available mouthrinses against strains of C. albicans and R. mucilaginosa. METHODS: The six mouthwashes studied in vitro were formulated (alone or in combination) with chlorhexidine (CHX) 0.12%, CHX 0.1%, CHX 0.05%, cetylpyridinium chloride (CPC) 0.075%, CPC 0.05%, and essential oils. Ten C. albicans and R. mucilaginosa isolates each were studied. The agar diffusion method (Mueller Hinton II), with incubation at 32°C was used to evaluate the antifungal activity. RESULTS: The results of this study indicate that mouthwashes with CHX 0.1%, CHX 0.12%, CHX 0.05% + CPC 0.05%, CHX 0.12% + CPC 0.05% and CPC 0.075% have an antifungal effect against C. albicans and R. mucilaginosa. CHX 0.1% led to the broadest inhibition zone for C. albicans and R. mucilaginosa (25.65±2.39mm and 40.05±3.31mm). Essential oils did not show any antifungal activity. Statistical analysis showed no statistical difference between mouth rinses CHX 0.1%, CHX 0.12% and CHX 0.12% + CPC 0.05% (p=0.0001) against C. albicans and R. mucilaginosa. CONCLUSIONS: Mouthwashes with CHX showed higher antifungal activity against C. albicans and R. mucilaginosa than other mouthwashes studied.

Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: un estudio in vitro / Antifungal activity of mouthwashes against Candida albicans and Rhodotorula mucilaginosa: an in vitro study

ANTECEDENTES: Candida albicans y Rhodotorula mucilaginosa son levaduras de importancia clínica en la cavidad oral. En pacientes inmunocomprometidos estas levaduras pueden producir enfermedades que deben ser controladas con antimicrobianos. OBJETIVOS: Evaluar y comparar la eficacia antifúngica de seis enjuagues bucales comerciales frente a aislamientos de C. albicans y R. mucilaginosa. MÉTODOS: Fue evaluada in vitro la eficacia de seis enjuagues bucales formulados (solos o en combinación) con clorhexidina (CHX) 0,12%; CHX 0,1%; CHX 0,05%; cloruro de cetilpiridinio (CPC) 0,075%; CPC 0,05% y aceites esenciales. Fueron utilizados diez aislamientos de C. albicans y otros diez de R. mucilaginosa. Mediante el método de difusión en placa con agar Mueller Hinton (modificado) se midieron los halos de inhibición previa incubación a 32°C. RESULTADOS: Los resultados de este estudio indican que enjuagues bucales con CHX 0,1%; CHX 0,12%; CHX 0,05% + CPC 0,05%; CHX 0,12% + CPC 0,05% y CPC 0,075% ejercen un efecto antifúngico frente a C. albicans y R. mucilaginosa. CHX 0,1% dio lugar a la mayor zona de inhibición para C. albicans y R. mucilaginosa (25,65±2,39mm y 40,05±3,31mm). El enjuague con aceites esenciales no tuvo actividad antifúngica alguna. El análisis estadístico no mostró diferencia entre los enjuagues bucales CHX 0,1%; CHX 0,12% y CHX 0,12% + CPC 0,05% (p = 0,0001) frente a C. albicans y R. mucilaginosa. CONCLUSIONES: Los enjuagues bucales con CHX mostraron una mejor actividad antifúngica contra C. albicans y R. mucilaginosa que los restantes enjuagues estudiados

BACKGROUND: Candida albicans and Rhodotorula mucilaginosa are yeasts of clinical importance in the oral cavity. In immunocompromised patients they can cause some pathologies that must be controlled with antimicrobials. AIMS: To evaluate and compare the antimicrobial efficacy of commercially available mouthrinses against strains of C. albicans and R. mucilaginosa. METHODS: The six mouthwashes studied in vitro were formulated (alone or in combination) with chlorhexidine (CHX) 0.12%, CHX 0.1%, CHX 0.05%, cetylpyridinium chloride (CPC) 0.075%, CPC 0.05%, and essential oils. Ten C. albicans and R. mucilaginosa isolates each were studied. The agar diffusion method (Mueller Hinton II), with incubation at 32°C was used to evaluate the antifungal activity. RESULTS: The results of this study indicate that mouthwashes with CHX 0.1%, CHX 0.12%, CHX 0.05% + CPC 0.05%, CHX 0.12% + CPC 0.05% and CPC 0.075% have an antifungal effect against C. albicans and R. mucilaginosa. CHX 0.1% led to the broadest inhibition zone for C. albicans and R. mucilaginosa (25.65±2.39mm and 40.05±3.31mm). Essential oils did not show any antifungal activity. Statistical analysis showed no statistical difference between mouth rinses CHX 0.1%, CHX 0.12% and CHX 0.12% + CPC 0.05% (p = 0.0001) against C. albicans and R. mucilaginosa. CONCLUSIONS: Mouthwashes with CHX showed higher antifungal activity against C. albicans and R. mucilaginosa than other mouthwashes studied